Takes place in 1526 China at the beginning of the decline of the Ming Dynasty. You play as Shao Jun, the last member of the Chinese Brotherhood, who returns to her homeland. Trained by the legendary Ezio Auditore, she is determined to seek revenge and restore the Brotherhood.

1841. The tension between the Sikh Empire and the East India Company reaches its peak. When one of the top Na'ites arrives with a mysterious object that belonged to the Assassin Brotherhood, Arbaaz Mir must uncover its purpose, steal the object, and protect his friends and loved ones.

1918. Nikolai Orelov wants to leave with his family, but he must carry out one last mission for the Brotherhood. He must infiltrate the house where the Bolsheviks are holding the Tsar's family captive and steal an object sought after by both the Assassins and the Templars. He will witness the murder of the Tsar's children, but he will manage to save Princess Anastasia. He will have to escape from the Templars while protecting the object and Anastasia.